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. 2020 May 4;9(5):43. doi: 10.1038/s41389-020-0227-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Binucleate assay: a Giemsa staining; representative optical microscope images, magnitude 20×. b % number of binucleate cells for 200 nuclei counted; n = 3; c Cell cycle analysis by flow cytometry, right: cell cycle distribution of analyzed cells, percentage. 10,000 singlets (n = 3). Left: a representative image of each condition is shown. d Cell cycle analysis for phenotype rescue experiments. Left: representative images of each condition are shown. C1: clone 1; C2: clone 2. Totally, 10,000 singlets, (n = 3). Down: qRT-PCR expression of miR-93 after miR-93 mimic transfection vs. scramble. Right: effects of miR-93 overexpression in PANC-1 KO-miR-93 cells represented as the percentage of increase in G2/M cells with respect to PANC-1 control cells transfected with scramble miR in both KO-miR-93 clones transfected with scramble or miR-93 mimick. Error bars: s.d. *p < 0.05 **p < 0.01 ***p < 0.001.