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. 2020 May 4;11(5):308. doi: 10.1038/s41419-020-2502-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a The protein levels after siRNA treatment relative to the effect of a scrambled siRNA. Quantitation performed using fluorescent secondary antibodies and Licor™ scanner. b Live-cell imaging of caspase-3 activity using the Incucyte. C2C12 cells transfected with different siRNAs (100 nM) were incubated with a cell permeable substrate (5 µM) and images were captured every 2 h for 48 h. The data were normalized to the maximum fluorescence intensity of the control siRNA at 48 h and expressed as the mean ± SD of three independent experiments (*P < 0.05). Percent inhibition of myosin heavy chain expression (c) and cell fusion (d) by the different siRNAs. The data are the mean ± SD of three independent experiments.