Fig. 7. TGCT copy number signatures.

Five copy number signatures (CN-Sig-1, CN-Sig-3, CN-Sig-4, CN-Sig-5, CN-Sig-6) identified using TCGA SNP array data (n = 105). a Defining features of the CN signatures, showing each feature (SegSize, osCN, ChangePoint, BP10, CopyNumber and BPChr) split into 36 constituent components, as defined in ref. ²⁷. The mean value for each component is shown on the x axis, with component weights shown on the y axis. Features are defined as follows: SegSize, segment size (Mb); ocCN, region length with neighbouring oscillating copy number segments (Mb); ChangePoint, difference in copy number between neighbouring segments; BP10, number of break points (10 Mb⁻¹), CopyNumber, absolute copy number of segment; BPChr, breakpoints per chromosome arm. b CN signature exposure correlations with clinical and molecular features. Two-sided Pearson’s correlations for association of q < 0.05 are illustrated. Age, aneuploidy and chromosome 12p copies: analysed across histologies. RAS pathway-related features: analysed in seminomas only. c Box plots showing CN signature exposure comparisons for KIT (n = 12) and RAS (n = 7, NRAS/KRAS) mutated seminomas, wildtype seminomas (n = 18) and nonseminomas (n = 66). Boxes show the median ± 25–75th percentiles, whiskers show 1.5× interquartile range below and above the 25th and 75th percentiles, respectively. p values are derived from two-sided Wilcoxon Rank Sum tests adjusted for multiple comparisons via the Benjamini-Hochberg method.