Table 2.
Values of inhibitory concentrations (IC50) and Combinatory Index of the phenothiazinium combinations.
| Combination (compound 1 + 2) |
IC50 (μM) (compound 1) |
IC50 (μM) (compound 2) |
IC50 (μM) (combination in relation to compound 1) |
IC50 (μM) (combination in relation to compound 2) |
CI |
|---|---|---|---|---|---|
| NMB + MB | 0.069 ± 0.006 | 0.221 ± 0.048 | 0.021 ± 0.008 | 0.119 ± 0.05 | 0.84 |
| DMMB + MB | 0.024 ± 0.013 | 0.394 ± 0.109 | 0.017 ± 0.012 | 0.249 ± 0.146 | 1.36 |
| NMB + DMMB | 0.021 ± 0.002 | 0.064 ± 0.018 | 0.016 ± 0.002 | 0.040 ± 0.004 | 1.40 |
| NMB + Pyr | 0.072 ± 0.012 | 0.347 ± 0.044 | 0.053 ± 0.018 | 0.301 ± 0.077 | 1.60 |
| DMMB + Pyr | 0.029 ± 0.012 | 0.436 ± 0.184 | 0.026 ± 0.016 | 0.377 ± 0.159 | 1.78 |
The IC50 concentrations of the dyes alone and in combinations were calculated using the Compusyn software66. The software was also used for the determination of the CI between the combined compounds.