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. 2020 May 4;11(5):309. doi: 10.1038/s41419-020-2511-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative flow images of CD4⁺ CD25⁺ FoxP3⁺ T cells taken from B16 tumors treated as above indicated. b, c Statistics of the percentage of CD4⁺T cells, CD8⁺ T cells, FoxP3⁺ T cells and ratio of CD8/FoxP3 in the B16 or MC38 tumors. d Representative images of CD31 immunostaining (red), 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (blue) and markers of immune cells (CD4, CD8, and FoxP3) (green) of B16 tumors (n = 5 per group, except for Bev group n = 4) treated as above indicated. Scale bars,100 mm. e Quantification of CD4⁺ T cells, CD8⁺ T cells, FoxP3⁺ T cells in B16 tumors. f Statistics of perivascular and without perivascular CD8⁺ T cells in the B16 tumors. g Statistics of ratio of CD8/FoxP3 in the B16 or MC38 tumors. All data are exhibited as mean ± SD. Statistical differences were assessed using the unpaired Student’s test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.