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. 2020 May 4;10:7429. doi: 10.1038/s41598-020-64117-x

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© The Author(s) 2020

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HQ formation in dsDNA upstream of a T7 promoter. (a) Scheme of HQ formation in a transcribed DNA. (b) Detection of HQ formation in four DNAs using native gel electrophoresis. HQ formation is shown by the extra DNA band (blue arrowhead) migrating behind the original DNA. N, No transcription. T, Transcribed. Asterisk indicates a fluorescein label at the 5’ end.