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. 2020 May 4;10:7487. doi: 10.1038/s41598-020-64556-6

Table 1.

Behavioural taste responses of blue gourami Trichopodus trichopterus to pellets of agar gel (2%) flavoured with free amino acids or their derivatives (0.1 М).

Taste substances Latent period, s Pellet consumption, % Index of palatability Number of grasps over the entire trial Pellet retention time, s: Number of trials
after the first grasp over the entire trial
L-α-Alanine 4.27 ± 0.42 92.0 ± 3.9*** 31.4 1.20 ± 0.06*** 7.74 ± 0.50*** 8.51 ± 0.46*** 50
D-α-Alanine 3.62 ± 0.43* 40.8 ± 7.1 −8.1 2.49 ± 0.23 4.71 ± 0.66 8.03 ± 0.79** 49
L-β-Alanine 3.35 ± 0.23 25.5 ± 6.2* −30.6 2.16 ± 0.19 2.74 ± 0.28 4.71 ± 0.56 51
Glycine 4.46 ± 0.36 34.0 ± 6.8 −17.1 1.82 ± 0.16 4.45 ± 0.51 5.94 ± 0.54 50
Glycyl-glycine 3.68 ± 0.30 20.0 ± 5.7** −41.2 2.40 ± 0.27 1.95 ± 0.24*** 3.50 ± 0.42* 50
Methyl-glycine (=sarcosine) 5.08 ± 0.87 20.0 ± 5.7** −41.2 2.20 ± 0.22 2.51 ± 0.30 4.30 ± 0.53 50
Dimethyl-glycine-HCl 5.40 ± 1.05 25.5 ± 6.2* −30.6 2.25 ± 0.22 3.16 ± 0.45 7.99 ± 0.86* 51
Trimethyl-glycine (=betaine) 4.23 ± 0.91* 30.0 ± 6.5 −23.1 2.24 ± 0.22 3.80 ± 0.26 6.25 ± 0.78 50
Glycolic acid 5.36 ± 0.51 74.0 ± 6.3** 21.3 1.88 ± 0.23 5.78 ± 0.65** 7.77 ± 0.71** 50
Acetic acid 4.68 ± 0.51 10.0 ± 4.3*** −65.5 2.94 ± 0.40 1.57 ± 0.16*** 4.09 ± 0.73** 50
L-Cysteine 4.25 ± 0.76 28.0 ± 6.4* −26.3 3.08 ± 0.33* 3.19 ± 0.44 5.21 ± 0.52 50
L-Cystine 4.64 ± 0.70 66.0 ± 6.8 15.8 2.40 ± 0.40 5.83 ± 0.62*** 8.71 ± 0.66*** 50
Mosquito  larvae extracta 4.63 ± 0.56 100.0 ± 0.00*** 35.1 1.14 ± 0.07*** 7.41 ± 0.39*** 7.72 ± 0.40*** 51
Control (blank pellets) 5.15 ± 0.55 48.0 ± 7.1 2.16 ± 0.19 3.28 ± 0.39 4.89 ± 0.44 50

aConcentration of water extract of mosquito larvae in pellets = 175 g. l−1. Values are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001 relative to control (χ2 test – for pellet consumption; the Mann-Whitney U test – for others).