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. 2020 May 4;10:7455. doi: 10.1038/s41598-020-64418-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Oocytes are unable to efficiently target cyclin A1 for degradation. (A) Frames from live cell imaging experiment of oocytes co-injected with cRNAs encoding histone (red) and either cyclin A2 or cyclin A1 (green) fused to fluorescent proteins. Scale bar represents 10 μm. (B) Profiles of fluorescent signal of cyclin A1 (orange, n = 12) and cyclin A2 (blue, n = 7) during meiotic maturation. The curves represent average curve for each construct and the standard deviation error bars are shown. The signal in each cell was normalized to the frame closest to the disassembly of the nuclear membrane (GVBD). The data were obtained from two independent experiments. (C) Profiles of fluorescent signal of securin fused to fluorescent protein in oocytes injected by cyclin A1 (orange, n = 14) or cyclin A2 (blue, n = 10) cRNAs during meiotic maturation. The curves represent average expression curve for all cells in the group and the standard deviation error bars are shown. The signal in each cell was normalized to the frame closest to GVBD. The data were obtained from two independent experiments.