Table 1.

Expression of cyclin A1 transcript in comparison to cyclin A2 and cyclin B1 transcripts is dramatically lower in oocytes than in spermatocytes.

Average Ct for indicated transcripts
	cyclin A1	cyclin A2	cyclin B1
GV oocyte	38.36 ± 0.89	27.47 ± 0.63	25.63 ± 0,36
Spermatocyte	30.38 ± 1.16	26.78 ± 0.53	26.08 ± 0.89
	ΔCt for indicated transcripts	ΔCt (cyclin A1 – cyclin A2)	ΔCt (cyclin A1 – cyclin B1)
	GV oocyte	10.89	12.73
	Spermatocyte	3.60	4.30

Expression levels of cyclin A1, cyclin A2 and cyclin B1 mRNA were analyzed in GV oocytes and spermatocytes using qPCR and TaqMan probes. The threshold cycle (Ct) for each transcript is indicated. The relative expression of cyclin A1 to cyclin A2 and cyclin B1 was calculated as a difference between the Cts for each transcript.