Fig. 5.

Replication capacities of clade B wild type and Rev Q101stop mutated virus in PM1 cells. A Diagrams of the mutation in clade B Rev. Red character suggests the mutated base or amino acid. B Replication capacities of virus mutants in PM1 cells. PM1 (7 × 10⁵) cells were infected with 10 ng p24 antigen clade B wild type (AT1) or mutated virus (AT1-Q101Stop) virus in 1 mL total volume, then cells were washed twice by 10 mL PBS and seeded in 2 mL fresh medium. Supernatant (0.5 mL) was substituted by fresh medium at days 3, 5 and 7 post infection for p24 antigen quantification. Data are representative of at least three independent experiments. Statistical difference was presented as ***P < 0.001.