Fig. 2.

Weak base NH₄Cl could inhibit GCRV entry and infection. A Fluorescence distribution of QD-bio-GCRVs infected cells in the presence or absence of NH₄Cl. Cells were incubated with or without 10 mmol/L NH₄Cl prior to exposure to QD-bio-GCRV at an MOI of 100 at 28 °C. Imaging was performed at 3 hpi by the laser confocal microscope. B–C Viral proteins expression and virus yield assays in NH₄Cl-treated CIK cells. Cells were pretreated with different drugs at the indicated concentrations and then infected with GCRVs in the presence of drugs. The expression level of viral proteins was analyzed by WB (B), and the progeny virus titer was determined by plaque assay (C). Three independent experiments, repeated three times for each sample, were performed. Asterisks denote a statistically significant change in virus titer compared to that in the absence of NH4Cl (*P < 0.05; **P < 0.01).