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. 2020 Apr 28;8:284. doi: 10.3389/fcell.2020.00284

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Copyright © 2020 Sarmento, Hof and Šachl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Basic principles of MC-FRET for detection of nanodomains. (A) At a sufficiently high acceptor concentration in the bilayer, FRET between donors (green stars) and acceptors (red stars) occurs. (B) If donors and acceptors with high affinity for nanodomains are used, the presence of nanodomains leads to accumulation of donors and acceptors in the nanodomains, and consequently to a more efficient FRET. (C) The efficiency of FRET on a homogeneous vs a heterogeneous membrane with nanodomains.