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. 2020 Apr 28;8:308. doi: 10.3389/fcell.2020.00308

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Copyright © 2020 Lupo, Piro, Torroni, Delfino, Trovato, Rusev, Fiore, Filippini, De Sanctis, Manfredi, Marengo, Lawlor, Martini, Tortora, Ugel, Corbo, Melisi and Carbone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of obesity on organoid-transplant models engraftment and growth. (A) Obesity accelerated the engraftment rate of preinvasive preneoplastic (mP) and neoplastic (mT) organoid models. Engraftment rate was represented as percentage of mice with pancreatic cysts respect to all mice of each group. (B) Ultrasound imaging of pancreatic cysts; the volume was reports as the increase of external epithelium (red line). (C) Volume analysis of pancreatic cysts. Data of each group were reported as mean and 95%CI; (D) cystic neoplasm closure. All the measures were taken with ultrasound technology (VEVO 2100). *, p < 0.05; ***, p < 0.001; by two-tailed unpaired Student’s t-tests. The borders distance of each cyst was measured with ImageJ software; Red line, external epithelium; Blue line, internal epithelium.