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. 2020 Apr 28;8:308. doi: 10.3389/fcell.2020.00308

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Copyright © 2020 Lupo, Piro, Torroni, Delfino, Trovato, Rusev, Fiore, Filippini, De Sanctis, Manfredi, Marengo, Lawlor, Martini, Tortora, Ugel, Corbo, Melisi and Carbone.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Obesity affected the immune infiltrate in organoid –transplant systems. Representative immunohistochemical staining for Ki67, CD11b, CD8, and LY6G positive cells in tissues from mice bearing (A) mP and (B) mT organoid-derived cells. Scale bars, 100 μm. The experiment was performed in three model of each group. (C) Quantification of paraffin-embedded tumor sections stained immunohistochemically with antibodies against Ki67, CD11b, CD8, and LY6G. Number of positive cells were from ten different area with pancreatic lesions. The mean values and standard deviation are shown. ***, p < 0.001; *, p < 0.05 by two-tailed unpaired Student’s t-tests.