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. 2020 Apr 28;8:308. doi: 10.3389/fcell.2020.00308

FIGURE 5.

FIGURE 5

Obesity-induced carcinogenesis and progression of mT organoids models are associated with changes of gene expression profile. (A) RNA-Seq based PCA showing a homogeneous cluster of LFD mice, while HFD and ob/ob ones display a more heterogeneous profile. (B) Heatmap of the differentially expressed genes from mT of obese and lean mice. Top 30 genes with an adjusted p-value < 0.05 are reported. (C) Boxplots showing the RNA-Seq based GSVA scores of different custom gene sets (LFD, n = 3, HFD, n = 3, ob/ob n = 2). (D) The panel represents the RNA-Seq based subtyping for each individual mT mice of the three groups. A sample was assigned to a Bailey, Collisson or Moffitt (Stroma subtypes separated) subtype when its GSVA score was highest within that subtype group. LFD mice are clearly more homogenous than HFD and ob/ob. (E) Results of the pre-ranked GSEA applied to the results of the differential gene expression between the three pairs indicated. Only gene sets with an adjusted p-value < 0.05 are displayed.