TABLE 2.
Phenotypic features used as readout markers for the characterization of in vitro polarized neutrophils.
| N1 | N2 | References | Assay |
| technique | |||
| CD54high | CD54low | (3) | Flow cytometry |
| CD95high | CD95low | (3) | Flow cytometry |
| CD182low | CD182high | (61, 62) | Flow cytometry |
| TNF production high | TNF production low | (5) | ELISA |
| IP-10 production high | IP-10 production low | (5) | ELISA |
| IL-8 production low | IL-8 production high | (3) | ELISA |
| H2O2 production high | H2O2 production low | (6) | Luminol assay |
The cited references refer to literature describing the differences between N1 and N2 TANs in murine tumor models. H2O2, hydrogen peroxide; IL, interleukin; IP-10, interferon-γ-induced protein 10; TANs, tumor-associated neutrophils; TNF, tumor necrosis factor.