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. 2020 Apr 28;8:245. doi: 10.3389/fcell.2020.00245

FIGURE 1.

FIGURE 1

NEFA induces oxidative stress and apoptosis in dairy cow hepatocytes. (A) cell survival of hepatocytes treated with various NEFA concentrations (0, 0.3, 0.6, 1.2, or 2.4 mM) for 4 h was determined via the CCK-8 assay. (B) Cytotoxicity of hepatocytes treated with various NEFA concentrations (0, 0.3, 0.6, 1.2, or 2.4 mM) for 4 h was measured using an LDH assay kit. (C) The GSH content, (D) the MDA content, and (E) intracellular ROS production in dairy hepatocyes incubated with different concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM) without or with NAC (10 mM). (F) Cellular apoptosis in dairy hepatocyes incubated with different concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM) were stained with Hoechst 33258 and were observed under a fluorescence microscope. The data are presented as the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 vs. the control group. #p < 0.05 and ##p < 0.01 vs. 2.4 mM NEFA-treated group.