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. 2020 Apr 28;8:245. doi: 10.3389/fcell.2020.00245

FIGURE 2.

FIGURE 2

Effect of NEFA on the apoptosis signaling pathway in hepatocytes. (A) Apoptotic cells analysis in dairy cows hepatocytes incubated without or with NEFA (2.4 mM) in the absence or presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). The protein expression levels of (B) ERK and (C) JNK in dairy cows hepatocytes treated with various concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM). The protein expression levels of (D) ERK and (E) JNK in dairy cows hepatocytes incubated without or with NEFA (2.4 mM) in the absence or presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). Quantification of (F) p-ERK/ERK and (G) p-JNK/JNK (n = 5). *p < 0.05 and **p < 0.01 vs. the control group. #p < 0.05 and ##p < 0.01 vs. 2.4 mM NEFA-treated group.