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Effect of NEFA on the phosphorylation of ERK and JNK in hepatocyte at different time points. Hepatocytes were treated with 2.4 mM NEFA at 0, 0.5, 1, 2, 4, 6, and 24 h. Expression of (A) phosphorylation-ERK (p-ERK), ERK, phosphorylation-JNK (p-JNK) and JNK were evaluated by western blot. Quantification of (B) p-ERK/ERK and (C) p-JNK/JNK (n = 5). *p < 0.05 and **p < 0.01 vs. the control group.
