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. 2020 Apr 28;8:245. doi: 10.3389/fcell.2020.00245

FIGURE 5.

FIGURE 5

Effect of NEFA on apoptosis-related genes. (A) The protein expression levels of caspase 3, cleaved caspase 3, caspase 9, PARP, C-PARP, apaf-1, and ASK1 in dairy cows hepatocytes treated with various concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM). (B) The protein expression levels of caspase 3, cleaved-caspase 3, caspase 9, PARP, C-PARP, apaf-1, and ASK1 in dairy cows hepatocytes incubated without or with NEFA (2.4 mM) in presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). Quantification of (C) cleaved-caspase 3/caspase 3, caspase 9, C-PARP/PARP, apaf-1, and ASK1 in the absence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). Quantification of (D) cleaved-caspase 3/caspase 3, caspase 9, C-PARP/PARP, apaf-1, and ASK1 in the presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). All results were obtained from three independent experiments and are expressed as the mean ± SD. *p < 0.05 and **p < 0.01 vs. the control group.