FIGURE 7.

Effect of NEFA on the activation of p53. (A) The protein expression levels and (B) quantification of p53 in dairy cows heaptocytes incubated with different concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM). (C) The protein expression levels and (D) quantification of p53 in dairy cows heaptocytes incubated with different concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM) in the presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). (E) The transcriptional activity of p53 in dairy cows hepatocytes treated with different concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM) in the presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). (F) The NEFA-induced translocation of p53 from the cytosol to the nucleus in dairy cows hepatocytes incubated without or with NEFA (2.4 mM) in the presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). Hepatocytes were fixed, stained with a specific antibody against p53 (red fluorescence) and counterstained with Hoechst 33258 (blue fluorescence). All results were obtained from three independent experiments and are expressed as the mean ± SD. *p < 0.05 and **p < 0.01 vs. the control group.