FIGURE 8.

NEFA induce the inhibition of Nrf2 in dairy cow hepatocytes. (A) The protein expression levels and (B) quantification of Nrf2 in dairy cows heaptocytes incubated with different concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM). (C) The protein expression levels and (D) quantification of Nrf2 in dairy cows hepatocytes incubated with different concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM) in the presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). (E) The transcriptional activity of Nrf2 in dairy cows treated with different concentrations of NEFA (0, 0.3, 0.6, 1.2, or 2.4 mM) in the presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). The NEFA-induced translocation of Nrf2 from the cytosol to the nucleus in dairy cows hepatocytes incubated without or with NEFA (2.4 mM) in the absence or presence of SP600125 (10 μM), PD98059 (10 μM), and NAC (10 mM). Hepatocytes were fixed, stained with a specific antibody against Nrf2 (red fluorescence) and counterstained with Hoechst 33258 (blue fluorescence). All results were obtained from three independent experiments and are expressed as the mean ± SD. *p < 0.05 and **p < 0.01 vs. the control group.