Figure 5.

Knockdown of SOX8 attenuated drug resistance in JEG3 chemoresistant sublines. (A) Western blotting analysis on protein expression of SOX8 following lentivirus-mediated shRNA knockdown in JEG3 sublines. β-actin was used as loading control. (B) IC₅₀-values of chemotherapeutic drugs in JEG3 chemoresistant sublines expressing Scr or shSOX8 shRNAs. n = 3, *P < 0.05. (C) Knockdown of SOX8 impaired soft agar clonogenesis after drug treatment (MTX: 10 μg/mL; 5-Fu: 50 μg/mL; VP16: 10 μg/mL) in JEG3 sublines. The colony formation in Scr group without drug treatment was regarded as 100%, respectively. n = 4, *P < 0.05. (D) Knockdown of SOX8 increased caspase-3 activity following drug treatment (MTX: 10 μg/mL; 5-Fu: 50 μg/mL; VP16: 10 μg/mL) for 48 h in JEG3 sublines. The caspase-3 activity in Scr group without drug treatment was regarded as 100%, respectively. n = 4, *P < 0.05.