Figure 6.

Over-expression of SOX8 promoted drug resistance in GTN cell lines. (A) Lentiviral transduction of SOX8 considerably increased their protein expression in JEG3 and JAR cells. β-actin was used as loading control. (B) IC₅₀-values of chemotherapeutic drugs in JEG3 and JAR cells expressing SOX8 or EV. n = 3, *P < 0.05. (C) Over-expression of SOX8 rescued soft agar clonogenesis after drug treatment (MTX: 1 μg/mL; 5-Fu: 5 μg/mL; VP16: 2 μg/mL) in JEG3 and JAR cells. The colony formation in EV of each cell line without drug treatment was regarded as 100%, respectively. n = 4, *P < 0.05. (D) Over-expression of SOX8 attenuated caspase-3 activity following drug treatment (MTX: 1 μg/mL; 5-Fu: 5 μg/mL; VP16: 2 μg/mL) for 48 h in JEG3 and JAR cells. The caspase-3 activity in EV of each cell line without drug treatment was regarded as 100%, respectively. n = 4, *P < 0.05.