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. 2020 Apr 28;10:557. doi: 10.3389/fonc.2020.00557

Figure 7.

Figure 7

Attenuation of ROS induced by drugs might be associated with drug resistance in GTN cells. (A) ROS levels of JEG3 and JEG3 chemoresistant sublines after drug treatment. JEG3 and JEG3 sublines were treated with MTX (1 μg/mL), 5-Fu (5 μg/mL), or VP16 (2 μg/mL) for 48 h, respectively. ROS levels in JEG3 without drug treatment were regarded as 100%. n = 4, *P < 0.05. (B) Cytotoxicity induced by drugs was associated with ROS accumulation in JEG3 cells. JEG3 cells were treated with MTX (1 μg/mL), 5-Fu (5 μg/mL), or VP16 (2 μg/mL) alone or with NAC (5 mM) for 48 h, followed by CCK-8 assay to assess the cell viability. Cell viability in JEG3 without drug treatment was regarded as 100%. n = 4, *P < 0.05. (C) Cytotoxicity induced by drugs was associated with ROS accumulation in JAR cells. JAR cells were treated with MTX (1 μg/mL), 5-Fu (5 μg/mL), or VP16 (2 μg/mL) alone or with NAC (5 mM) for 48 h, followed by CCK-8 assay to assess the cell viability. Cell viability in JAR without drug treatment was regarded as 100%. n = 4, *P < 0.05.