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. 2020 Apr 28;10:557. doi: 10.3389/fonc.2020.00557

Figure 8.

Figure 8

SOX8 regulated the expression of antioxidant enzymes and reduced the drug-induced ROS accumulation in GTN cells (A) Knockdown of SOX8 increased drug-induced ROS in JEG3 sublines. JEG3 sublines expressing Scr or shSOX8 shRNAs were treated with MTX (10 μg/mL), 5-Fu (50 μg/mL), or VP16 (10 μg/mL) for 48 h. The DCFDA fluorescence in Scr group of three JEG3 sublines without drug treatment was regarded as 100%, respectively. n = 4, *P < 0.05. (B) Over-expression of SOX8 attenuated drug-induced ROS in GTN cell lines. GTN cells (JEG3, JAR) expressing EV or SOX8 were treated with MTX (1 μg/mL), 5-Fu (5 μg/mL), or VP16 (2 μg/mL) for 48 h. The DCFDA fluorescence in EV group of JEG3 or JAR cells without drug treatment was regarded as 100%, respectively. n = 4, *P < 0.05. (C) Knockdown of SOX8 reduced the expression of antioxidant enzymes GPX1 and HMOX1 in JEG3 chemoresistant sublines. The gene expression of GPX1 or HMOX1 in Scr group of three JEG3 sublines was regarded as 100%, respectively. n = 3, *P < 0.05. (D) over-expression of SOX8 increased GPX1 and HMOX1 expression in JEG3 and JAR cells. The gene expression of GPX1 or HMOX1 in EV group of JEG3 or JAR cells was regarded as 100%, respectively. n = 3, *P < 0.05.