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. 2020 Apr 28;11:786. doi: 10.3389/fmicb.2020.00786

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Copyright © 2020 Ohbayashi, Hirooka, Onuma, Kanesaki, Hirose, Kobayashi, Fujiwara, Furusawa and Miyagishima.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ploidy of the chromosome of Synechococcus sp. PCC 7002. Exponentially growing Synechococcus sp. PCC 7002 (chromosome, approximately 3.0 Mbp) and for comparison, S. elongatus (chromosome, approximately 2.7 Mbp; 3–6 copies per cell; Zheng and O’Shea, 2017) (Supplementary Figure S2) were fixed and stained with SYBR Green and then examined using flow cytometry. (A) SYBR Green-stained cells. Images were acquired using differential interference contrast microscopy (top) and fluorescence microscopy (bottom). (B) Distribution of the DNA levels per cell and cell volumes of exponentially growing cultures of Synechococcus sp. PCC 7002 (black) and S. elongatus (blue). (C) The relationship between the DNA level and size of Synechococcus sp. PCC 7002.