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. 2020 Apr 28;7:190. doi: 10.3389/fvets.2020.00190

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Copyright © 2020 Cui, Xu, Zhou, Xu, Wang, Xiao, Li and Bi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histopathology in liver, heart, kidney, and spleen from dead chickens at 5 to 7 dpi (stained with HE) inoculated with CH/HBTF/1710. Massive pathological damage was observed in the virus-inoculated chickens. Degeneration, vacuolar necrosis, and basophilic inclusion bodies were present in hepatic cells (A–C). Rupture and necrosis of myocardial fibers were observed in heart tissue (E–G). Swelling and degeneration of renal tubular epithelial cells were present in the kidneys (I–K). Severe reduction and necrosis of lymphocytes were seen in the spleen (M–O). There was no significant histopathological damage present in the liver (D), heart (H), kidney (L), and spleen (P) of chickens from the control group. Scale bar = 200 μm in (A,B), and 100 μm in the other panels. Solid arrows point to lesion areas.