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. 2020 Apr 28;11:763. doi: 10.3389/fimmu.2020.00763

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Copyright © 2020 Lin, Tai, Brož, Tang, Chen, Wu, Li and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of glycolysis by 2-deoxy-D-glucose (2DG) treatment resulted in decreased ATP levels and increased AcMNPV replication in BmN cells and larvae. The ATP level was measured at 48 h postinfection (hpi) after 2DG treatment in BmN cells (A) and larvae (C); the values were normalized to those in non-infected control BmN cells and BmNPV-infected larvae, respectively. Virus titers were estimated at 48 hpi in BmN cells (B) and larvae (D); BmNPV treatment was used as the positive control. All values are shown as the mean ± SEM of four replicates for ATP measurements and three replicates for virus titer. Significance was determined by one-way ANOVA with Tukey's HSD post-hoc analysis; different letters for the treatment group indicate significant differences at P < 0.05.