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. 2020 Apr 28;11:763. doi: 10.3389/fimmu.2020.00763

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Copyright © 2020 Lin, Tai, Brož, Tang, Chen, Wu, Li and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AdoR RNAi suppressed ATP induction and increased AcMNPV titers in BmN cells. (A) Knockdown efficiency of AdoR siRNA treatment in BmN cells by RT-PCR analysis. (B) The ATP level was measured at 48 h postinfection (hpi) in AdoR and control siRNA-treated cells; values were normalized to those in the non-infected control. Virus titers were determined by qPCR (C) and fluorescence intensity (D) at 48 hpi in AdoR- and control siRNA-treated cells. All values are the mean ± SEM of four replicates for ATP level and three replicates for virus titer measurements. Significance was determined by one-way ANOVA with Tukey's HSD post-hoc analysis; different letters for the treatment group indicate significant differences at P < 0.05.