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. 2020 Apr 28;11:512. doi: 10.3389/fphar.2020.00512

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Copyright © 2020 Jiang, Jiang, Wang, Ma and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quercetin alleviated human aortic endothelial cells (HAECs) senescence in vitro. HAECs were cultured for 48 h in the presence of 50 μg/mL ox-LDL, or 50 μg/mL ox-LDL followed with different quercetin (3, 1 or 0.3 μmol/L), and untreated HAECs was used as normal control. (A) HAECs senescence was assessed by SA-β-G staining. (B) Subcellular morphology was observed under transmission electron microscopy (TEM). (C) Fluorescence images of mitochondria by Mito Tracker Green as molecular probe. (D) HAECs senescence rate was semi-quantitative analyzed according to SA-β-G staining. *P < 0.05, vs untreated HAECs; ^ΔP < 0.05, vs ox-LDL; n = 3.