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. 2020 Apr 28;10:183. doi: 10.3389/fcimb.2020.00183

Figure 1.

Figure 1

Cloning of tyeA gene. M: DL2000 marker. Lane 1-5:The 285bp fragment was amplified from genomic DNA of the wild-type strain HY9901 using primer pairs of tyeAP1-for/tyeAP1-rev (Lane 1, 2, 3, 4, 5:Tm = 56°C, 57°C, 58°C, 59°C, 60°C).