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Comparative analysis of the expression of immune-related genes in liver and spleen of zebrafish given the live attenuated vaccine and unvaccinated zebrafish. The head kidney and spleen of grouper were sampled at 1 day before challenge, and the mRNA level of each immune-related gene was normalized to that of β-actin. Bars represent the mean relative expression of three biological replicates and error bars represent standard deviation.
