Table 1.
Bacterial strains, plasmids, and experimental fish used in this study.
| Strains, plasmids | Relevant characteristics | Source |
|---|---|---|
| V.alginolyticus HY9901 | Wild type, isolated from diseased Lutjanus sanguineus off the Southern China coast | (Cai et al., 2007) |
| E. coli DH5α | supE44 ΔlacU169 (ϕ80 lacZDM15) hsdR17 recA1 gyrA96 thi-1 relA1 | TakaRa |
| MC1061 (λpir) | lacY1 galK2 ara-14 xyl-5 supE44 λpir | (Rubirés et al., 1997) |
| pRE112 | pGP704 suicide plasmid, pir dependent, oriT, oriV, sacB, C mr | (Edwards et al., 1998) |
| S17-1 (λpir) | T prSmrrecA thi pro hsdR-M+RP4:2-Tc: Mu: K m T n7 λpir | (Simon et al., 2019) |
| S17-1-pRE-ΔtyeA | S17-1 containing plasmid of pRE-ΔtyeA, C mr | This study |
| pMD18-T | Cloning vector, Ampr | TakaRa |
| Zebrafish | experimental fish, purchased from Zhanjiang Aquatic Market | Zhanjiang Aquatic Market |