Table 4.

Comparison of biological characteristics between HY9901 and HY9901ΔtyeA.

Characteristics	HY9901	HY9901ΔtyeA
Activity of ECPase(A422)a	1.03 ± 0.2	0.97 ± 0.2
Biofilm thickness(μm)b	60 ± 10	90 ± 20
Swarming (mm)c	34.9 ± 0.2	20.6 ± 0.4**
LD50(CFU mL−1)d	5.8 × 105	2.6 × 107**

Values are mean ± standard deviation for three trials. Significant differences between HY9901 and HY9901ΔtyeA indicated by asterisk.

^**p < 0.01.

^aBacteria were incubated in TSB for 18 h at 28°C.

^bBacteria were incubated in a glass bottom culture dish (NEST) for 24 h at 28°C.

^cSwarming diameters were measured after 24 h incubation on TSA containing 0.3% agar plates.

^dLD₅₀were evaluated in healthy zebrafish with an average weight of 0.2 g.