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. 2020 Apr 28;8:318. doi: 10.3389/fchem.2020.00318

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Copyright © 2020 Darabedian, Yang, Ding, Cutolo, Zaro, Woo and Pratt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ac₄2AzMan labels a wide variety of intracellular proteins. H1299 cells were treated with either Ac₄2AzMan (200 μM) or DMSO vehicle for 16 h. Labeled proteins were then enriched using neutravidin beads after CuAAC with alkyne-biotin. Proteins were then identified using label free quantitation after on-bead trypsinolysis and LC-MS/MS. The results are shown as a Volcano Plot (x-axis: log₂ ratio of MCR to vehicle, y-axis; –log₁₀ p-value). Significantly enriched proteins that differ at least 5 linear-fold with a p < 0.01 (Student's t-test) are marked in red.