Figure 3.

Abrogating pUS28 expression does not impact US27 or US29 transcription. NuFF-1 fibroblasts were infected (moi = 0.5) with (A) BFXwt-based or (B) TB40/EmCherry-based viruses. Total RNA was harvested 96 hpi and US27, US28, US29, UL123, and UL99 mRNA levels were quantified by RTqPCR. Viral gene expression is plotted relative to cellular GAPDH. Each data point (circles) is the mean of three technical replicates (e.g., one biological replicate). Error bars indicate standard deviation of three biological replicates, and statistical significance was calculated using two-way ANOVA analyses followed by Tukey's post-hoc analyses. *p < 0.05; ns, not significant.