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. 2020 Apr 28;10:186. doi: 10.3389/fcimb.2020.00186

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Copyright © 2020 Krishna, Wass, Sridharan and O'Connor.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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US28Δ-infected fibroblasts display wild type pUS27 levels. NuFF-1 fibroblasts were mock-infected (M) or infected with TB40/EmCherry (WT), TB40/EmCherry-vGPCRmulti, or TB40/EmCherry-vGPCRmulti-US28Δ (moi = 0.5). At 96 hpi, (A) cell lysates were collected for immunoblot or (B) harvested for IFA. (A,B) α-FLAG was used to detect US28 via the 3xF epitope, α-HA was used to detect US27 via the 3xHA epitope. (A) IE1 is shown as a marker of infection, and cellular actin serves as a loading control. (B) US27-3xHA (green), US28-3xF (white), mCherry (red) serves as a marker of infection. DAPI (blue) was used to visualize nuclei. Images were acquired using 40x objective. (A,B) Representative images are shown (n = 3).