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. 2020 Apr 28;14:274. doi: 10.3389/fnins.2020.00274

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Copyright © 2020 Jin, Jiang, Luan, Qu, Guo, Gao, Xu and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of Orexin-A on food intake in rats. After injection of orexin-A into CeA, the chow food intake of the rats did not change during the 0–2 h (A) period and 2–4 h (A). The high fat food intake of the rats increased during the 0–2 h (B) period after micro-injection of orexin-A, but this phenomenon did not appear in 2–4 h (B). SB-334867 completely and SCH-23390 partially blocked the action of orexin-A. Data are represented as mean ± SD, n = 6. Data were analyzed using one-way ANOVA followed by a Bonferroni post hoc test to compare the effect of different orexin-A groups and student’s t-test was used to determine the effect of SB-334867 and SCH-23390. *p < 0.05, **p < 0.01 vs. NS group; ^#p < 0.05 vs. 0.5 μg orexin-A group. ^@p < 0.01 vs. 0.05 μg orexin-A group. NS, normal saline.