Figure 4.

PF attenuates H₂O₂-induced oxidative stress in melanocytes. Melanocytes were pretreated with PF for 24 h and exposed to 1.0 mM H₂O₂ for another 24 h. (A) The ROS production were analyzed by the flow cytometry. The activities of antioxidant enzymes SOD (B) and CAT (C) were respectively detected with Catalase Assay kit and Total Superoxide Dismutase Assay kit with WST-8 assay. All data were presented as the mean ± standard deviation across three independent experiments. **P < 0.01, ***P < 0.001. ns, not significant; ROS, reactive oxygen species; SOD, superoxide dismutase; CAT, catalase; H₂O₂, hydrogen peroxide; PF, Paeoniflorin; h, hours; M, mol/L.