Figure 6.

PF activates JNK/Nrf2/HO-1 pathway in H₂O₂-treated melanocytes. Melanocytes were treated with PF for 24 h and further treated with H₂O₂ for another 24 h. (A) Western blots of total Nrf2, p-Nrf2, and its downstream antioxidant enzymes, NQO1, and HO-1. (B) The protein levels of JNK and p-JNK, (C) The levels of Nrf2 and HO-1 proteins in melanocytes treated with SP600125 and PF followed by H₂O₂. The quantitative analysis of Nrf2, p-Nrf2, NQO1, and HO-1 (A), JNK and p-JNK (B), and Nrf2 and HO-1 (C) using Tublin as normalization were showed at the right, respectively, all data are presented as mean ± standard deviation. n = 3 per group, *P < 0.05, **P < 0.01, ns, not significant; H₂O₂, hydrogen peroxide; PF, Paeoniflorin; M, mol/L; h, hours; JNK, c-Jun N-terminal kinase. p-, phosphorylated.