Figure 3.
Kaempferol potentiates angiogenesis in vivo. Healthy zebrafish embryos were picked out for each group and placed onto a 12-well plate with density set at 8–10 embryos/well. Embryos were fed with PU water containing 5 ng/ml VEGF with or without kaempferol or 200 μg/ml Avastin on 1st day of development. After drug treatment for 48 h, the fish embryos were stained. The blood vessels located in the sub-intestinal were captured for blood vessel formation. Images represented the basket of sub-intestinal vessels of the zebrafish embryos developed for 3 days. The branches and area of sub-intestinal vessels in control group and medicine-treated group were quantified with application of Image J software. Results are shown as the percentage of change as compared to control (no drug) in mean ± SEM, where n = 3; p < 0.05 *p < 0.01 **p < 0.001 (***) vs VEGF-treated group. Bar = 40 μm.