Figure 6.

Kaempferol potentiates VEGF-triggered phosphorylations of eNOS and Erk. 20 × 10⁴ HUVECs were seeded into each well of a 12-well plate. VEGF (5 ng/ml) was determined with or without kaempferol or Avastin (200 μg/ml). The lysates of endothelial cells were collected at different time intervals, as demonstrated. Phosphorylated and total proteins of eNOS (A) and Erk (B) were separately analyzed by Western blotting. Right panel is the quantitation values deriving from left panel. Quantitation was done at different time, as well as different drug treatments, at 10 min of activation. Results are expressed as the fold of change as compared to control (X Basal), where the control group (no drug) was set as 1, mean ± SEM, where n = 3; p < 0.05 *p < 0.01 **p < 0.001 (***) vs VEGF-treated group. eNOS, endothelial nitric oxide synthase; Erk, extracellular signal-regulated kinase.