Reagent or Resource	Source	Identifier
Antibodies
Anti-GFP antibody	Abcam	Cat# ab6556; RRID: AB_305564
Anti-RFP antibody	Chromotek	Cat# 6g6-100; RRID: AB_2631395
Actin antibody	Sigma-Aldrich	Cat# A0480; RRID: AB_476670
Anti-YFP antibody	Custom made	N/A
Pierce protein A magnetic beads	Thermo Scientific	88846- #NK80758
Goat anti-mouse	Jackson	Cat# 115-036-003; RRID: AB_2338518
Anti-rabbit	Jackson	Cat# 111-036-003; RRID: AB_2337942
Chemicals
24-Epibrassinolide (BL)	Sigma-Aldrich	E1641
Experimental Models: Organisms/Strains
bri1-5	[25]	N/A
bri1-6 (Enkheim-2)	[25]	N/A
bri1-301	[50]	N/A
pBRI1::BRI1-GFP	[26]	N/A
bzr1-d	[44]	N/A
pDR5rev::GFP	[14]	N/A
pDR5rev::mRFP1er	[41]	N/A
p35S::PILS2-GFP, p35S::GFP-PILS3	[21]	N/A
p35S::PILS5-GFP, p35S::PILS6-GFP	[21]	N/A
p35S::PILS5-GFP;pDR5rev::mRFP1er,	[21]	N/A
pils2-1pils5-2	[21]	N/A
pPILS2, 3, and 5::GFP/GUS-NLS, pils3-1	[23]	N/A
DII-VENUS and mDII-VENUS	[36]	N/A
DII-VENUS;PILS6OE and mDII-VENUS;PILS6OE	[24]	N/A
pils2-1 pils3-1 pils5-2	This study	N/A
p35S::PILS3-RFP pDR5rev::GFP;pils2-1 pils3-1 pils5-2	This study This study	N/A N/A
imp1;PILS5OE	This study	N/A
imp1	This study	N/A
imp1;PILS5OE;BRI1::BRI1-GFP	This study	N/A
pPILS5::GFP/GUS-NLS;pBRI1::BRI1-GFP	This study	N/A
pPILS5::GFP/GUS-NLS;bri1-5	This study	N/A
pPILS5::GFP/GUS-NLS;bri1-6	This study	N/A
pPILS5::GFP/GUS-NLS;imp1	This study	N/A
pPILS5::GFP/GUS-NLS;bzr1-d	This study	N/A
bri1-6;PILS5OE	This study	N/A
bri1-301;PILS5OE	This study	N/A
Recombinant DNA		N/A
p35S::PILS3-RFP;pK7RWG2	[21]	N/A
Software and Algorithms		N/A
Graph Pad Prism5	http://www.graphpad.com	N/A
Leica SP5 or Leica SP8	https://www.leica-microsystems.com/	N/A
ImageJ	https://imagej.net	N/A