Figure 2.

Layer V Increases the Precision of Encoding of Whisker Deflection Time

(A and B) Representative pseudo-simultaneous population response to whisker stimulation (red bar, A) for the neurons displayed in Figures 1E and 1F, in the absence (A) or presence of layer V photoactivation (blue bar, B).

(A₁ and B₁) Number of spikes per neuron for the same single trials shown in (A) and (B). The dashed line indicates the threshold (Th) for population response event (PRE) detection (see STAR Methods). Gray asterisks indicate PREs.

(C and D) Same as (A) and (B), respectively, for whisker-responsive neurons displayed in Figures 1H–1J.

(C₁ and D₁) Same as (A₁) and (B₁) for the same trials shown in (C) and (D).

(E and E₁) PREs in all pseudo-simultaneous whisker stimulation trials for the same neurons shown in (A) and (B) in the absence (E) or presence of layer V photoactivation (blue bar, E₁).

(F and F₁) Same as in (E) and (E₁) for layer V photosuppression.

(G) Absolute estimate time error of PREs (absolute error) following whisker deflection and whisker deflection in the presence of layer V photostimulation. n = 99 (no layer V activation), n = 39 (layer V activation at ~0 ms), n = 59 (layer V activation at ~30 ms), and n = 65 (layer V activation at ~50 ms) PREs from n = 13 neurons from 7 animals; Kruskal-Wallis test.

(H) Same as in (G) for layer V photoinhibition. n = 138 (no layer V inactivation) and n = 237 (layer V inactivation) PREs from n = 19 neurons from 14 animals; Mann-Whitney test.

See also Figure S4.