Figure 3.

Ever-Decreasing Proliferation Rate of DPs during Postnatal Growth

(A) Protocol of the EdU/BrdU double-labeling experiments.

(B–G) Representative P4 whole-mount tail epidermis at the given time points (B and C) (maximum-intensity projection of confocal images) and higher magnification of BCs in interscale (D and F) and scale (E and G) at the same time points.

(H–M) Representative P15 whole-mount tail epidermis at the given time points (H and I) (maximum-intensity projection of confocal images) and higher magnification of BCs in interscale (J and L) and scale (K and M) at the same time points. Yellow arrowheads, EdU/BrdU double-labeled cells; white circles, HFs. Nuclei are stained with Hoechst. Scale bars, 50 μm.

(N) Quantification of EdU/BrdU double-positive BCs in scale and interscale (n ≥ 3 mice). Symbols, experimental data; lines, theoretical fits from a model of stochastic division after a refractory phase. Data are represented as mean ± SEM.

(O) Time evolution of the division rate in scale (red) and interscale (blue) BCs from P1 to P30. Symbols, experimental data; dashed lines, fit of the decrease in division rate over time, used for subsequent clonal modeling; error bars are mean ± SD.

(P) Average division time (TDiv) of epidermal BCs in interscale and scale, calculated from EdU/BrdU analysis.

(Q) Quantification of BrdU+ primary keratinocytes isolated from P2, P10, P30, and P60 mice, cultured for 48 h and incubated for 2 h with BrdU (n = 2 experiments). Error bars are mean ± SD.

See also Figure S2.