Figure S6.

Single-Cell RNA Sequencing of DPs in Young Samples and Stem/Progenitors in Adult Samples, Related to Figure 6

(A-B) Heatmap showing the relative expression of the genes defining the different populations in young (A) and adult (B) samples. DP G0 and SC/CP G0 populations are shown with arrows. (C-G) UMAP dimensionality reduction plots colored by the degree of regulon activation for transcription factors differentially activated (AUC rank-sum test FDR corrected p value < 0.05) in DP G0 I (C) and DP G0 II (D) or both (E) and in differentiated cells (F,G) in young (upper panel) and adult (lower panel). Color scaling:AUC value of target genes in the regulon being expressed as computed by SCENIC. (H) Heatmap showing the relative expression of the genes identified in adult SC/CP populations in non-cycling BCs at P7, P30 and P60. Rows represent marker genes for P60 SC/CP subpopulations with a log-fold change in expression greater than 0.3. Columns represent cells belonging to DP clusters at P7 and SC/CP clusters at P30 and P60. The color of the cells represent normalized expression values for each gene-cell combination. P7 and P60 samples were subsampled to 200 cells each respectively. (I-K) Scatterplots of marker gene set enrichment. Dots represent individual cells in DP clusters at P7 (I) and SC/CP clusters at P30 (J) and P60 (K). The x axis represents the AUC values computed for each cell using the AUCell package and describe how high they express the P60 SC/CP G0 II marker genes whereas the y axis represents AUC values for the SC/CP G0 III marker genes. Linear correlations between the two AUC values is linked to sensitivity of detection and is correlated with the number of detected genes. Cells following a diagonal trend represent cells that homogeneously express the two sets of markers while cells deviating from the trend represent cells expressing distinct transcriptional marker gene signatures.