Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 28;11:357. doi: 10.3389/fphys.2020.00357

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Flatt, Stevens-Hernandez, Cogan, Eggleston, Haines, Heesom, Picard, Thomas and Bruce.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Immunocytochemistry of permeabilized homozygous SAO erythrocytes. Circulating erythrocytes from the homozygous SAO individual were fixed, permeabilized and imaged using immunofluorescent labeling and confocal microscopy. Antibodies directed against intracellular epitopes of plasma membrane proteins. Mouse monoclonal antibodies were used to detect band 3 (BRIC170, BRIC155, BRIC132), stomatin (GARP50), ankyrin (BRIC274) and alpha-spectrin (BRIC174). In-house rabbit polyclonal antibodies were used to detect GPC, CD44 and phospho-Tyr359 band 3. Scale bars represent 5 μm.