Skip to main content
. 2020 Mar 4;34:101492. doi: 10.1016/j.redox.2020.101492

Fig. 4.

Fig. 4

Preventing RS (by withdrawing SF) rescues myoblast differentiation. Myoblasts were treated with SF (5 μM) during proliferation and differentiation phases until day 3. Removal of SF (washout) on day 3 by replenishing cells with fresh differentiation medium and allowed to differentiate until day 5. (A) Bright field images of proliferating and differentiating myoblast until day 5 under SF or rescued SF treatment. (B) Western blots of markers of myoblast differentiation and antioxidant proteins in proliferating and differentiating myoblasts. (C) Densitometry analysis of NQO1, myogenin, GCLC and MYH2 normalized with GAPDH was presented in bar graphs. *p < 0.05 and ***p < 0.001, n = 3–4.