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. Author manuscript; available in PMC: 2020 May 5.
Published in final edited form as: Biochemistry. 2018 Sep 18;57(39):5696–5705. doi: 10.1021/acs.biochem.8b00730

Table 4.

Thermodynamic Properties of Substrate Binding to EFE

substrate ΔG (kcal/mol) δΔH (kcal/mol) −TΔSa (kcal/mol)
iron (binding to EFE) −9.6b −11.8 ± 0.7b +2.2
2OG (binding to Fe·EFE) −7.2b −13.0 ± 2.8c +6.8
Arg (binding to Fe·EFE) −5.6d +10.0 ± 3.8c −15.6
2OG (binding to Fe·EFE·Arg) −6.26d −38.0 ± 2.8c +31.74
Arg (binding to Fe·EFE·2OG) −6.1d −15.0 ± 2.6c +8.93
a

Calculated from ΔG and δΔH values reported here using the equation ΔG = δΔH − TΔS, where the δΔH term is estimated to be equal to the enthalpy of binding.

b

Value determined from ITC experiments shown in Table S8 and Figure S4

c

Thermodynamic parameters derived from DSC data collected in this study.

d

Calculated values based on the Kd reported by Martinez et al.18